开展海洋动物包括鱼、虾、蟹和海参等的细胞培养及其基因转移技术研究，主要研究内容：

（1） 对虾、红鳌螯虾和三疣梭子蟹等甲壳动物细胞的体外2D和3D长期培养与建系研究；

（2） 对虾及其细胞的高效基因转移技术研究，包括对虾病毒（IHHNV和CMNV）介导的基因转移与表达技术的构建与应用；

（3） 鱼类细胞的体外培养与建系，以及细胞培养鱼肉关键技术研究；

（4） 鱼类和鱼类细胞的体内和体外毒理学研究；

（5） 珊瑚和砗磲细胞及其共生藻的体外培养与生态系统修复研究。

郭华荣    教授 博士生导师

山东省细胞生物学学会理事会常务理事

E-mail: huarongguo@ouc.edu.cn

Tel: 15963279730

教育及工作经历：

1997-2001,中国科学院海洋研究所，理学博士.

1994-1997,中国科学院海洋研究所，理学硕士.

1988-1992，青岛海洋大学海洋生命学院海洋生物学专业，理学学士。

1992-1994，青岛海洋大学教师。

2001-至今，中国海洋大学海洋生命学院，先后任讲师（2001-2002）、副教授（2002-2012）、教授（2013.12）。2018年1月获聘中国海洋大学博士生导师。主授本科生《细胞工程》(双语课)和《细胞工程前沿讲座》，以及研究生课程《细胞工程》（中文/英文）。

2004.11，参加中泰科技合作项目：海产经济动物配子及胚胎（种质）冷冻保存，访问泰国东方大学,为期10天。

2005.1-5，赴美国University of Illinois at Urbana-Champaign做访问学者，参加国家留学基金委组织的双语教师培训项目，并参与了哺乳动物细胞的mTOR信号转导研究。

2008.8-2009.2, 赴美国University of South Carolina 生物系做博士后研究，主要从事哺乳动物细胞的蛋白质磷酸酶与p53信号转导通路研究。

主要研究方向及成果

本人所领导的研究团队一直致力于对虾、螃蟹、鱼类、珊瑚和水母等海洋动物的细胞和组织的体外培养技术研究。尤其在对虾成体和胚胎组织细胞的体外培养条件的优化与建立以及病毒介导的嗜对虾基因转移技术的建立方面独树一帜，成绩斐然，在国内外处于领先水平。今后，研究团队将矢志不渝地探究对虾细胞体外生长停滞的分子机制，建立对虾永生性细胞系，开发高效的对虾基因编辑系统，为对虾病毒病的防治以及对虾功能基因的研究提供有效的研究工具与技术平台。

近年来主持国家自然科学基金面上项目2项；参与国家重点研发项目“蓝色粮仓科技创新”专项1项；主编双语教材《Cytotechnology/细胞工程技术》1部，参编教材和专著3部；在Aquaculture和Aquatic Toxicology等权威期刊发表SCI论文30余篇；获国家发明专利5项和实用新型发明专利1项；获国家海洋局海洋创新成果一等奖1 项（第2位）；获天泰优秀人才奖3次和本科教学优秀奖1次。

代表性文章

1. Xuemei Chen, Yueru Chen, Xiaotong Shen, Jianwei Zuo, Huarong Guo*（2019）. The improvement and application of lentivirus-mediated gene transfer and expression system in penaeid shrimp cells. Marine Biotechnology, 21: 9-18.

2. Jing Wang#, Yuqi Lin#, Xin Song#, Longjun Pu, Guangdong Ji, Guorong Li, Huarong Guo*（2019）. Promoter of shrimp translationally controlled tumor protein (TCTP) gene has higher activity than cytomegavirus (CMV) promoter in driving foreign genes to express in primary shrimp cells. Crustaceana, 92 (9) 1039-1061.

3. Longjun Pu, Jing Wang, Xiaojuan Zhang, Huarong Guo* (2017). Development of pseudotyped retroviral system for effective gene transfer and expression in penaeid shrimp cells. Aquaculture, 467, 198-210.

4. Cuicui Liang, Yuhao Wang, Yujie Wang, Huarong Guo* (2017). Telomerase reverse transcriptase gene from greasyback shrimp Metapenaeus ensis: isolation, genomic organization and expression pattern analysis.  Zoological Science, 34(5), 419-428.

5. Li Han, Ya-Nan Zhai, Yang Liu, Linhua Hao*, Huarong Guo* (2017). Comparison of the In Vitro and In Vivo Toxic Effects of three Sizes of Zinc Oxide (ZnO) Particles using Flounder Gill (FG) Cells and Zebrafish Embryos. Journal of Ocean University of China，16（1），93-106.

6. Qiongxuan Lu, Tao Liu, Xianming Tang, B0 Dong*, Huarong Guo*(2016). Reformation of tissue balls from tentacle explants of coral Goniopora lobata: self-organization process and response to environmental stresses. In Vitro Cell.Dev.Biol.—Animal, 53(2), 111-122.

7. Qian Han, Dandan Dong, Xiaojuan Zhang, Cuicui Liang, Qiongxuan Lu, Huarong Guo* (2015). Problems with the use of liposome- and retrovirus-mediated gene transfer methods in the primary lymphoid cells of the Oka organs of the greasyback shrimp, Metapenaeus ensis (De Haan, 1844). Crustaceana, 88 (12-14), 1351-1365.

8. Manish Raj Pandey, Huarong Guo*（2015）. Evaluation of cytotoxicity and genotoxicity of insecticide carbaryl to flounder gill cells and its teratogenicity to zebrafish embryos. J. Ocean Univ. China, 14 (2), 362-374.

9. Manish Raj Pandey, Huarong Guo*(2014). Evaluation of cytotoxicity, genotoxicity and embryotoxicity of insecticide propoxur using flounder gill (FG) cells and zebrafish embryos. Toxicology in Vitro, 28(3), 340-353.

10. Qian Han, Pengtao Li, Xiongbin Lu, Zijuan Guo, Huarong Guo*（2013）. Improved primary cell culture and subculture of lymphoid organs of the greasyback shrimp Metapenaeus ensis. Aquaculture 410-411,101-113.

11. Deyu Geng, Zhixia Zhang, Huarong Guo*（2012）. Development of a Fish Cell Biosensor System for Genotoxicity Detection Based on DNA Damage-Induced Trans-Activation of p21 Gene Expression. Biosensors2(3), 318-340.

12. Fan Yang, Qianqian Zhang, Huarong Guo*, Shicui Zhang^* (2010). Evaluation of cytotoxicity, genotoxicity and teratogenicity of marine sedimentsfrom Qingdao coastal areas using in vitro fish cell assay, comet assayand zebrafish embryo test. Toxicology in Vitro24, 2003-2011.

13. Na Na,Huarong Guo*⁽2009). In vitro and in vivo acute toxicity of fenpyroximate to flounder Paralichthys olivaceus and its gill cell line FG. Aquatic toxicology 92, 76-85.

山东省重点研发计划（重大科技创新工程）项目：高品质细胞培养鱼肉关键技术创新集成与产品创制（2023-2026），子课题负责人.

国家自然科学基金项目：复制缺陷型对虾IHHNV病毒介导的高效基因转移与表达系统的构建与应用（2023-2026），主持.

山东省自然科学基金面上项目：对虾细胞球体外连续培养系统的构建及其应用（2021-2023），主持.

国家重点研发计划“蓝色粮仓科技创新”专项“重要养殖虾蟹类种质创制与健康苗种繁育”项目: 虾蟹类新种质创制技术平台（2018-2022），子课题负责人.

国家自然科学基金项目：嗜对虾细胞的高效病毒报告基因表达系统研究及其在转基因对虾上的应用（2015-2018），主持.

国家自然科学基金项目：对虾细胞的分子重编程与永生性转化研究（2012-2015），主持.

2024 Jing Wang^#， Longjun Pu^#，Xiaojuan Zhang，Cuicui Liang, Dandan Dong, Jiantao Guan，Huarong Guo*. Integrative analysis of transcriptomic profile reveals potential roles of miRNAs in regulating development in Marsupenaeus japonicus. Journal of Oceanology and Limnology, 42(1), 201-215.

2024 Yiwen Tao^#, Jinwu Wang^#, Rui Xiao, Qingli Zhang, Huarong Guo*. Development and evaluation of a shrimp virus (IHHNV)-mediated gene transfer and expression system for shrimps. International Journal of Molecular Sciences, 25, 8999.

2024 Yaqi Zhao, Ting Xue, Jinwu Wang, Huarong Guo*. Development of reliable in vitro long-term culture systems for hematopoietic cells of crayfish Cherax quadricarinatus and virus susceptibility assay. Aquaculture, 589 (2024), 741016.

2024 Liu Song, Yifu Xu, Yaqi Zhao, Jinwu Wang, Huarong Guo*. Identification of active shrimp U6 promoters for efficient CRISPR/Cas9 gene editing in penaeid shrimps based on a VP28-pseudotyped baculovirus expression system. Aquaculture 591(2024), 741128.

2024 Ting Xue^#, Yue Zhang^#, Zhenxin Zhao, Yaru Li, Jinwu Wang, Huarong Guo^*. Establishment and characterization of a continuous marine fish fin cell line from Epinephelus fuscoguttatus and viral susceptibility to crustacean CMNV. Journal of Oceanology and Limnology. doi: 10.1007/s00343-024-4054-4.

2023 Liwen Guo, Yaqi Zhao, Huarong Guo*. Long-term in-vitro culture and subculture of the hemocytes of swimming crab (Portunus trituberculatus). Journal of Oceanology and Limnology, 41（5）：1918-1939.

2023 Yaqi Zhao, Liwen Guo, Huarong Guo*. Routine development of long-term primary cell culture and finite cell line from the peripheral hemolymph of greasyback shrimp (Metapenaeus ensis) and virus susceptibility. Aquaculture, 563(2023), 739007.

2023 Meiling Sun^#, Mingdong Liu^#, Hong Shan^#, Kang Li^#, Peng Wang^#, Huarong Guo^#, Yaqi Zhao, Rui Wang, Yiwen Tao, Liuyan Yang, Ying Zhang, Xiaoming Su, Yunhui Liu, Chunyang Li, James Lin, Xiu-Lan Chen, Yu-Zhong Zhang*, Qing-Tao Shen*. Ring-stacked capsids of white spot syndrome virus and structural transitions with genome ejection. Science advances, 9（8）, eadd2796.

2023 Yiwen Tao, Qingli Zhang, Huarong Guo*. Improvement and applications of adeno-associated virus 2 (AAV-2) system for efficient gene transfer and expression in penaeid shrimp cells. Aquaculture Reports, 32 (2023) ：101700.

2023 Yaqi Zhao, Huarong Guo*. A novel medium for long-term primary culture of hemocytes of Metapenaeus ensis. MethodsX, 11 (2023), 102335.

2021 Mengxi Wu, Qian Hu, Yang Zhou, Huarong Guo*. Development of a VP28-pseudotyped baculovirus expression system for efficient gene transfer in penaeid shrimps. Aquaculture, 541: 736741.

2021 Na Li, Liwen Guo, Huarong Guo*. Establishment, characterization, and transfection potential of a new continuous fish cell line (CAM) derived from the muscle tissue of grass goldfish (Carassius auratus). In Vitro Cellular & Developmental Biology-Animal 57：912-931.

2019 Xuemei Chen, Yueru Chen, Xiaotong Shen, Jianwei Zuo, Huarong Guo*. The improvement and application of lentivirus-mediated gene transfer and expression system in penaeid shrimp cells. Marine Biotechnology, 21: 9–18.

2019 Jing Wang^#, Yuqi Lin^#, Xin Song^#, Longjun Pu, Guangdong Ji, Guorong Li, Huarong Guo*. Promoter of shrimp translationally controlled tumor protein (TCTP) gene has higher activity than cytomegavirus (CMV) promoter in driving foreign genes to express in primary shrimp cells. Crustaceana 92 (9): 1039-1061.

2024 郭华荣；陶奕文. 一种用于在对虾中递送和表达外源基因的对虾病毒表达系统（专利号：ZL202111656.209.X）. 国家发明专利.

2024 郭华荣；薛霆. 一种用于长期培养鱼类肌肉干细胞的培养基（专利号：202211627471.6）.国家发明专利.

2024 郭华荣；薛霆. 一种用于海水鱼类肌肉干细胞快速分化的分化诱导培养基（专利号：ZL202211615748.3）. 国家发明专利.

2024 郭华荣；薛霆。一种用于淡水鱼类肌肉干细胞快速分化的培养基及其应用（专利号：ZL202211616057.5）. 国家发明专利.

2024 Huarong Guo; Xin Song;Yang Zhou. Method for continuous culture of shrimp cells (美国专利号：US 11,873,509). 美国发明专利.

2024 Huarong Guo; Mengxi Wu. Pseudotyped insect baculovirus gene transfer system and pseudotyped baculovirus for shrimps, construction method and use thereof (美国专利号：US 12,110,497). 美国发明专利.

2023 郭华荣；郭莉文. 一种用于螃蟹细胞的长期培养和连续传代的培养基（专利号：ZL202210818244.5）. 国家发明专利.

2023 郭华荣；赵亚琦. 一种用于对虾细胞长期培养和连续传代的培养基（专利号：ZL202211127826.5）. 国家发明专利.

2021 郭华荣；毋梦茜. 一种用于对虾的假型昆虫杆状病毒基因转移系统、病毒及构建方法和应用（专利号：ZL202010196617.0）. 国家发明专利.

2020郭华荣；宋欣；周阳. 一种对虾细胞连续培养方法（专利号：ZL201910622127.X）. 国家发明专利.

2019 郭华荣；张晓娟；陈月如. 一种对虾胚胎细胞的分离与培养方法(专利号：ZL201610322759.0）. 国家发明专利.