郭华荣,博士,教授/博导
邮箱:huarongguo@ouc.edu.cn
电话:15963279730 (Cell phone)
承担课程:海德学院本科生《细胞工程学》(双语);生命学院本科生《细胞工程》(双语)、《生物技术大实验III》(即《细胞工程实验》)和《细胞工程前沿讲座》;生命学院研究生《细胞工程》(中/英文);三亚研究院研究生《细胞工程》。
学习工作经历:
1988~1992,青岛海洋大学海洋生命学院,学士,海洋生物学专业
1992-1994,青岛海洋大学总务处,团总支书记
1994~1997,中国科学院海洋研究所,硕士,海洋生物学专业
1997~2001,中国科学院海洋研究所,博士,海洋生物学专业
2008~2009, 美国南卡罗莱纳大学生物系,博士后
2001~至今,中国海洋大学海洋生命学院,教授/博导
2004.11,访问泰国东方大学,历时14天,“海产经济动物配子及胚胎(种质)冷冻保存” 合作研究
2005.01-05,美国伊利诺伊大学香槟分校,访问学者,双语教学培训
2023.7-8, 澳大利亚阿德莱德大学,历时15天,教育教学培训
主要科研领域:
主要从事海洋动物细胞培养及其基因转移技术研究。近年来,主持国家自然科学基金面上项目3项;参加国家重点研发计划“蓝色粮仓科技创新”专项1项;主编双语教材《Cytotechnology/细胞工程技术》1部,参编教材和专著3部;在Science advances、Aquaculture和Aquatic Toxicology等权威期刊发表SCI论文50余篇;获授权国家发明专利9件和美国发明专利1件;获2021年度山东省海洋科技创新奖一等奖1项(第1位);获国家海洋局海洋创新成果一等奖1 项(第2位);获天泰优秀人才奖3次和本科教学优秀奖1次;获2022年度中国海洋大学教师教学创新大赛二等奖1次;获山东省第九届教学成果奖(高等教育类)1项(第10位)。
代表性成果:(#共同第一;*通讯作者)
1. Meiling Sun #, Mingdong Liu #, Hong Shan #, Kang Li #, Peng Wang #, Huarong Guo #, Yaqi Zhao, Rui Wang, Yiwen Tao, Liuyan Yang, Ying Zhang, Xiaoming Su, Yunhui Liu, Chunyang Li, James Lin, Xiu-Lan Chen, Yu-Zhong Zhang*, Qing-Tao Shen*, 2023. Ring-stacked capsids of white spot syndrome virus and structural transitions with genome ejection. Science advances, 9 (8), eadd2796.
2. Yaqi Zhao, Liwen Guo, Huarong Guo*, 2023. Routine development of long-term primary cell culture and finite cell line from the peripheral hemolymph of greasyback shrimp (Metapenaeus ensis) and virus susceptibility. Aquaculture, 563(2), 739007. 563(2023), 739007.
3. Yiwen Tao, Qingli Zhang, Huarong Guo, 2023. Improvement and applications of adeno-associated virus 2 (AAV-2) system for efficient gene transfer and expression in penaeid shrimp cells. Aquaculture Reports, 32 (2023), 101700.
4. Yaqi Zhao, Huarong Guo, 2023. A novel medium for long-term primary culture of hemocytes of Metapenaeus ensis. MethodsX, 11 (2023), 102335.
5. Liwen Guo, Yaqi Zhao, Huarong Guo*, 2023. Long-term in-vitro culture and subculture of the hemocytes of swimming crab (Portunus trituberculatus). Journal of Oceanology and Limnology, https://doi.org/10.1007/s00343-022-2137-7.
6. Mengxi Wu, Qian Hu, Yang Zhou, Huarong Guo*, 2021. Development of a VP28-pseudotyped baculovirus expression system for efficient gene transfer in penaeid shrimps. Aquaculture, 541: 736741.
7. Na Li, Liwen Guo, Huarong Guo*, 2021. Establishment, characterization, and transfection potential of a new continuous fish cell line (CAM) derived from the muscle tissue of grass goldfish (Carassius auratus). In Vitro Cellular & Developmental Biology-Animal, 57:912-931.
8. Xuemei Chen, Yueru Chen, Xiaotong Shen, Jianwei Zuo, Huarong Guo*,2019. The improvement and application of lentivirus-mediated gene transfer and expression system in penaeid shrimp cells. Marine Biotechnology, 21: 9–18.
9. Jing Wang#, Yuqi Lin#, Xin Song#, Longjun Pu, Guangdong Ji, Guorong Li, Huarong Guo*, 2019. Promoter of shrimp translationally controlled tumor protein (TCTP) gene has higher activity than cytomegavirus (CMV) promoter in driving foreign genes to express in primary shrimp cells. Crustaceana 92 (9): 1039-1061.
10. Longjun Pu, Jing Wang, Xiaojuan Zhang, Huarong Guo*. 2017. Development of pseudotyped retroviral system for effective gene transfer and expression in penaeid shrimp cells. Aquaculture, 467: 198-210.