祁自忠(QI Zi-Zhong),男,1970年12月生,河南信阳人。理学博士,硕士生导师;中国海洋大学海洋生命学院高级工程师。
专业:海洋微生物学
研究方向:海洋微生物群落功能调控。包括有益微生物水产养殖中的应用、水产养殖病害防控、藻类共、附生菌群分析、菌群硝化功能和微生物海洋环境修复功能。
主要学术成就:
(1)应用海洋微生物群落组织与结构分析,阐明了海水养殖苗期食物链中有益微生物的作用,查清了不同营养水平对仿刺参养殖苗期微生物群落组织结构及机会致病菌群致病性的影响;系统分析了海水养殖开口饵料轮虫携带微生物菌群结构与功能特性;为水产养殖益生菌开发应用奠定了基础。
(2)在海洋微生物菌群硝化功能研究方面,以微生物指纹图谱、荧光原位杂交和高通量测序和功能基因分析技术,研究了海滨河口生态系统和海水养殖循环水系统下硝化和缺氧氨氧化菌群,初步查清了硝化和缺氧氨氧化菌群的种类多样性、组织分布和功能特征,以及营养元素对硝化功能的调控及其分子机理。
(3) 运用海洋细菌的富集培养和分离鉴定技术,研究了滨海沉积物和海底微生物燃料电池电极表面可培养细菌群落,重点分析了芽孢杆菌菌群的演替,分离得到了海洋细菌新种海洋底泥食冷菌Algoriphagus faecimaris;研究了海洋光合细菌的富集培养系统和微微型海洋浮游生物沉降系统,为海洋微生物资源开发利用奠定了基础。
(4)建立了基于膜分离技术的水产活饵料无菌养殖与实验系统。应用于高密度轮虫养殖、高品质轮虫筛选、以及大藻、微藻共附生细菌和活性物质的分离和作用研究。
学术成果应用:
依托海洋微生物组织与结构功能,开展规模化海洋微生物菌群相关产品的开发研究,主要产品包括海洋有益菌、饲料和添加剂活性成份、微生物肥活性酶、环保修复产品。与国内外相关企业战略合作,深入挖掘海洋微生物资源。
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一、学习与工作简历:
1991-1995年:中国海洋大学海洋生命学院,生态学与环境生物学专业,大学本科,学士学位。
1997-1999年:中国海洋大学海洋生命学院,海洋生物学专业,硕士研究生。
1999-2001年:比利时根特大学生物科学工程学院,水产科学专业,硕士学位。
2003-2008年:比利时根特大学生物科学工程学院,应用生物科学专业,博士学位。
1995-1999年:中国海洋大学海洋生命学院,海洋微生物实验室,助理工程师。
1995-1999年:联合国教科文组织中国海洋生物工程中心,管理助理。
2001-2002年:比利时根特大学生物科学工程学院水产与卤虫研究中心、微生物生态技术实验室,研究助理。
2003-2010年:中国海洋大学海洋生命学院,海洋遗传育种实验室、海洋微生物实验,工程师。
2010-现在: 中国海洋大学海洋生命学院,海洋微生物实验室,高级工程师。
二、主要研究课题和教学
(一)近年来的部分主要研究课题
2013.5-2015.5:企业研发项目:海参益生菌和功能饲料的开发。
2012.5-2013.5:企业研发项目:海参益生菌规模化发酵生产和应用。
2010.9-2012.9:比利时弗莱芒研究基金项目:“密度感应淬灭-新型水产养殖病害控制手段”。
2011.1-2013.12:国家自然科学基金项目“吲哚对迟缓爱德华氏菌耐药性系统作用机理的研究”(31072241)。
2009.01-2011.12:国家自然科学基金项目“长江口硝化、反硝化和缺氧铵氧化研究”(40876054)。
2009.01-2011.12:国家自然科学基金项目“海洋拮抗菌假交替单胞菌JG1作用机理的研究”(40876067)。
2008.01-2011.12:山东省科技发展计划项目“海底生物燃料电池阳极材料表面改性技术研究”(2008GG10007003)。
2006.01-2008.12:国家自然科学基金项目“参、鲍采苗板微生物群落的动态变化与苗期病害的关系”(30500377)。
2003.10-2008.12: 中国海洋大学与比利时大学发展委员会合作项目:“ 有益微生物调控水产育苗微生物区系(2003176)”。
2006.01-2008.12:国家863计划项目“深海环境通用自清洁防污涂层的研究”(2006AA09Z224)。
2007.01-2009.12:科技部支撑计划“浅海贝藻复合养殖技术集成与示范”(2006BAD09A09)。
2005.01-2010.12:国家海洋局908专项“ST02区块水体环境调查与研究”、“山东省908近海岸海域生物调查”微微型、微型浮游生物的研究。
2004.01-2006.12:国家自然科学基金项目“海洋弧菌中溶血素的研究”(30371119)。
(二)教学
系统主讲《微生物学》;讲授《微生物学》(双语)、《海洋微生物学》、《生物饵料学》、《潜水与海底生物调查》。主持校《潜水与海底生物调查》重点教学项目建设;参加校级精品课程《微生物学》建设;参加本科教育国际化课程《生物海洋学》建设。
(三)获奖和专利
2001年,欧盟项目“对虾苗期病害诊断与控制”国家海洋局海洋创新成果一等奖,第9名。
2006年,“养殖栉孔扇贝高产抗逆新品种培育”国家海洋局海洋创新成果一等奖,第4名。
2008年4月,以第三位获得国家发明专利,专利号ZL2006100421190。
2009年11月,以第三位获得国家发明专利,专利号ZL200710014430.9。
(四)主要代表论文
1. Zizhong Qi, Kristof Dierckens, Tom Defoirdt, Patrick Sorgeloos, Nico Boon, Zhenmin Bao,Peter Bossier. (2009) Effects of feeding regime and probionts on the diverting microbial communities in rotifer Brachionus culture. Aquacult Int 17:303–315. SCI
2.Zizhong Qi, Xiao-Hua Zhang, Nico Boon, Peter Bossier. (2009) Probiotics in aquaculture of China-Current state, problems and prospect. Aquaculture 290 15–21. SCI
3. Zizhong Qi, Kristof Dierckens, Tom Defoirdt, Patrick Sorgeloos, Nico Boon, Zhenmin Bao, Peter Bossier. (2009) Analysis of the evolution of microbial communities associated with different cultures of rotifer strains belonging to different cryptic species of the Brachionus plicatilis species complex. Aquaculture 292 23–29. SCI
4. 孙进,于敏,任晓亮,张月月,宋福来,祁自忠*(通讯)。2009(海藻叶面附着细菌群落生理特性与荧光原位杂交分析。海洋湖沼通报。4)51-61。核心。
5. Han, Y., Li, X., Qi, Z., Zhang, X.-H. and Bossier, P. (2010) Detection of different quorum sensing signal molecules in a virulent Edwardsiella tarda strain LTB-4. Journal of Applied Microbiology108:139-147. SCI
6. Yongxia Li, Shulin Yan, Qian Yang, Zizhong Qi*, Xiao-Hua Zhang, Yu-bin Fu. (Corresponding author). Algoriphagus faecimaris sp. nov., a novel bacterium isolated from Yellow Sea coastal sediment, China. Int J Syst Evol Microbiol first published on January 21, 2011 as doi: 10.1099/ijs.0.027862-0. SCI
7. 韩茵,李永霞,李杰,唐磊,祁自忠*。两种海洋细菌对海底沉积物微生物燃料电池产电效能及其阳极表面细菌群落的影响。海洋科学进展,2011,29:36-42.
三、国内外交流
参加欧盟项目“对虾对虾苗期细菌病害的诊断与控制”; 参与组织联合国教科文组织项目:“UNESCO中国海洋生物工程中心”1-4期国际培训课(主题:水产养殖动物病害;海产动物养殖技术;海藻及药物海洋生物;亚洲地区对虾养殖病害与生态防治)。参加和组织国际海洋生物工程进展与展望学术大会;参加国际学术大会与工作会议11次。与比利时根特大学、荷兰瓦赫宁根大学、挪威科技大学及越南、泰国、马来西亚、沙特阿拉伯等国同行保持良好合作关系;为亚欧水产科技平台和根特校友会成员。并以国家自然科学基金合作为基础,引进分子微生物学外国专家一名。
中国水产学会会员,山东省遗传学会会员,青岛欧美同学会会员。
联系方式:
Email: zizhongqi@ouc.edu.cn
中国海洋大学海洋生命学院
青岛市鱼山路5号
青岛266003
【对同学的期望语】以小见大,探索“看不见的海洋--微生物世界”。
Curriculum Vitae
Zizhong QI
Address: Yushan Road No. 5,
College of Marine Life Sciences, Ocean University of China
266003 Qingdao, China
E-mail: zizhongqi@ouc.edu.cn
Personal
Date of birth: 14th December 1970, Male
Place of birth: Xinyang, Henan, China
Nationality: Chinese
Education
1991-1995
Undergraduate study in Ocean University of Qingdao, College of Marine Life Sciences
Ecology
Excellent bachelor
Thesis: “Studies on the effect of different feeds on the health of larvae of Penaeus chinensis and the bacterial analysis in rearing water” performed at the Laboratory of Marine Microbiology. Promoter: Professor Huai-Shu Xu, Grade: A.
1999-2001
Master study in Ghent University, Faculty of Bioscience Engineering
Science in Aquaculture
Distinction
Thesis: “Using different formulations probiotics to improve the rotifer (Brachious plicatilis) culture” performed at the Laboratory of Microbial Ecology and Technology. Promoter: Professor Willy Verstraete, Grade: 15/20.
2003-2008
Doctoral study in Ghent University, Faculty of Bioscience Engineering/Ocean University of China, College of Marine Life Sciences
Applied Biological Sciences
Thesis: “The dynamics of microbial communities and probiotic supplements in the marine larviculture feed chain” performed at the Laboratory of Aquaculture and Artemia Reference Center, Laboratory of Microbial Ecology and Technology and Laboratory of Marine Genetic Breeding. Promoter: Professor Peter Bossier, Co-promoter Professor Nico Boon, Local promoter: Professor Zhenmin Bao.
Professional record
1995-1999
Research assistant at Laboratory of Marine Microbiology, Ocean University of Qingdao. Director: Prof. Huai-Shu Xu.
Administrative assistant of Chinese Center of Marine Biotechnology/BAC/UNESCO. Director: Prof. Huai-Shu Xu.
Participated in European Economic Community project: “Diagnosis and control of bacterial diseases in penaeid shrimp hatchery-relationships between microbial flora, nutrition, production techniques and health status of penaeid shrimp”.
Participated in National Natural Science Foundation of China (NSFC) project: “Marine biofouling and microbial control”.
Participated and assisted 4 times training courses of Marine biotechnology and aquaculture in Chinese Marine Biotechnology Center /BAC/UNESCO
Participated in the International Symposium on Progress and Prospect of Marine Biotechnolgy (ISPPMB’98).
Authored and co-authored 11 articles in local journals.
2002-present
Senior engineer at Laboratory of Marine Microbiology, Independent senior researcher.
•Doctoral research with Prof. dr. ir. P. Bossier (Laboratory of Aquaculture and Artemia Reference Center, Ghent University), Prof. dr. ir. N. Boon (Laboratory of Microbial Ecology and Technology, Ghent University) and Prof. Zhenmin Bao (Laboratory of Marine Genetics and Breeding, Ocean University of China) as scientific promoters. Research was supported by a grant of VLIR scholarship.
•Lecturing courses “Live food production” (2003), “Microbiology” (2008-2013) , “Marine Microbiology” (2009-2013) and “Diving and oceanic survey” for undergraduate students.
•Tutor of 21 undergraduate and 8 graduate students in biosciences during their graduated thesis (2003-2013).
•Collaborator in State Oceanic Administration project “Oceanic resource survey-picoplankton and nanoplankton” in North yellow sea and Qingdao coastal area.
•Collaborator in the Key technology R&D program of Ministry and Science and Technology “Integrated algae and shellfish cultures in shallow seawater-Integration and Demonstration”
•Collaborator in the High-tech program of Ministry and Science and Technology “Self-clean anti-fouling coat in deep sea environment”.
•Collaborator in 4 NSFC projects “Hemolysin in marine Vibrios”, “Functions of hemolysin in V. harveyi”,”CO2 concentration gene clone and expression in Arthrospira (Spirulina)” and “Respiratory Metabolism to Sulfide Exposure in Urechis unicinctus”
•Coordinator in NSFC project “The relationship of disease with the composition and dynamics of microbial communities on sea cucumber and abalone seeds settlement plates”
•Co-inventor of Chinese patent n°2006100421190 “A low mucosity algin producing method”.
Publications in recent five years (including international peer-reviewed journals):
Rombaut, G., Grommen, R., Qi, Z-Z., Vanhoof, V., Suantika, G., Dhert, P., Sorgeloos, P., Verstraete, W. (2003) Improved performance of an intensive rotifer culture system by using a nitrifying inoculum (ABIL). Aquaculture Research 34(2): 165-174.
LI, Y.,Yan, L-Y., Li, J., Qi, Z-Z,Zhang, X-H,Wang, X-H Xu, H-S. (2004) Study of heterotrophic bacterial flora,dominant Vibrios composition of larvae and postlarvae of Penaeus chinensis and its relationship with disease. Periodical of Ocean University of China. 34(6): 1003-1007 (in Chinese, Abstract in English).
Mu, C-H., Bao, Z-M., Cheng, G., Hu, J-J., Hao, L-J.,, QI, Z-Z., Li, G-X. (2005) Bacterial diversity in the sediments collected from the Shikoku Basin. Acta Oceanologica Sinica 24(3) 114-121.
Mu, C-H., Bao, Z-M., Chen, G., Hao, L-J, QI, Z-Z., Lao, J., Li, G-X., Hu J-J. (2006) Bacterial diversity in the sediments collected from the Northwest Pacific. Acta Oceanologica Sinica. 2006 (28) 4:121-128.
Bai, B., Pang, L., Qi, Z-Z., Chen J., Austin, B. and Zhang, X.-H. (2008) Distribution of five vibrio virulence-related genes among Vibrio harveyi isolates The Journal of General and Applied Microbiology 54: 71-78.
Qi, Z-Z., Dierckens, K., Defoirdt, T., Sorgeloos, P., Boon, N., Bao, ZM., Bossier, P. (2009) Effects of feeding regime and probionts on the diverting microbial communities in rotifer Brachionus culture. Aquaculture International 17: 303–315.
Qi, Z-Z., Defoirdt, T., Dierckens, K., Sorgeloos, P., Boon, N., Bao, ZM., Bossier, P. Analysis of the evolution of microbial communities associated with different cultures of rotifer strains belonging to different cryptic species of the Brachionus plicatilis species complex. Aquaculture 292: 23-29.
Qi, Z-Z., Zhang, X-H., Bossier, P. (2009) Current status and prospect of probiotics in aquaculture of China. Aquaculture 290: 15-21.
Sun, J., Yu, M., Ren, X-L., Zhang, Y-Y., Song, F-L, Qi, Z-Z. (2009) Ecophysiological characteristic and fluorescence in situ hybridization analysis of the epiphytic microbial community of Ulva lactuca and Undaria pinnatifida. Transactions of Oceanology and Limnology 4: 51-56.
Han, Y., Li, X., Qi, Z., Zhang, X.-H. and Bossier, P. (2010) Detection of different quorum sensing signal molecules in a virulent Edwardsiella tarda strain LTB-4. Journal of Applied Microbiology108:139-147. (SCI, IF2008: 2.028).
Li, Y.,Yan, S.,Yang, Q.,Qi, Z.*,Zhang, X. H.,Fu, Y. B. Algoriphagus faecimaris sp. nov., isolated from coastal sediment[J]. Int. J. Syst. Evol. Microbiol., 2011, 61 (12): 2856-2860.
HAN Yin,LI Yong-xia, LI Jie, TANG Lei, QI Zi-Zhong*. The Influences of two marine bacteria to the bacterial community compositions of the anode and the electricity production efficacy of marine benthic microbial fuel cell. Advances in Marine Science., 2011 29: 36-42.
Papers in Conference Proceedings
Z-Z. Qi, ZM. Bao, B. Sun, P. Sorgeloos, N. Boon, P. Bossier. 2005. Evaluating the effect of potential synbiotics on in vitro antagonism against a pathogen using DGGE. Larvi 2005 fish & Shelfish larviculture Symposium C.I. Hendry, G. Van Stappen, M. Wille and P. Sorgeloos (Eds). European Aquaculture Society Special publication no 36, Oostende, Belgium.
Workshops and course
Workshop: Recirculation in marine fish/shellfish larviculture. 14 Nov. 2003 Ghent university, ‘t Pand Onderbergen 1 Gent, Belgium.
Workshop: Environment & Ecosystem preservation, 23-26, April 2005, Qingdao, PR China.
Mini-symposium: Biofilm. 13 Nov. 2007. Ghent university, ‘t Pand Onderbergen 1 Gent, Belgium.
Course: Molecular workshop: Fluorescent in situ hybridization (FISH) training course, 5-6, June, 2007, Ocean University of China, Qingdao, P.R. China.
Research theme
Our research has focused on the microbial community of ocean, marine sediment, aquaculture systems, microbial fuel cells, intestinal ecosystems of marine animals and wastewater treatment systems. The areas of interests have been the using of molecular methods for the qualitative and quantitative description of opportunistic pathogens and investigations of microbial processes in live food production in aquaculture, novel symbiotic strategies for disease control and the selection and application of beneficial bacteria--probiotics. The research interests are focused on the analysis of microbial network to link the functionality of probiotics in vivo. The central theme of this research is to understand of the dominance of beneficial bacterial populations within a microbial community. This strategy is called Sustainable Probiotic Dominance (SPD).